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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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System components

Sample manager features

Sample manager features
Topic LCI-USG-0073, Last Updated 11/08/2024

The sample manager uses a direct injection mechanism to inject samples drawn from vials and plates onto a chromatographic column..

The Alliance iS Sample Manager maintains sample temperature, aspirates precise samples, and then injects them into the mobile phase using a flow-through-needle (FTN) design. The needle becomes part of the fluidics path when the sample is injected onto the column. The mobile phase cleanses the needle during the run, ensuring complete sample recovery and minimizing carryover. Additionally, the sample positioning mechanism controls the location of the sample plates relative to the sample needle, reducing extra system volume.

The standard configuration supports sample injection volumes of 0.1 to 100 μL. Optional extension loops can extend the injection volume up to 2000 μL. The sample temperature is regulated within a range of 4 °C (39.2 °F) to 40 °C (104 °F).
Note: A 100-µL extension loop is included in the standard configuration.

The sample manager features:

  • Three-plate sample capacity
  • Precise sample metering
  • Accurate sample temperature control
  • Exceptional carryover performance (0.002% maximum)
  • Consistent solvent consumption
  • Puncture robustness
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