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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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Priming the system

Priming the pump

Priming the pump
Topic LCI-USG-0011, Last Updated 11/08/2024

Priming the pump is part of the system startup workflow on the touchscreen.

Priming prepares a new system for use or for a change in reservoirs or solvents. It also prepares a system for restarting after it is idle for more than four hours. During priming, the vent valve moves to the vent position, allowing flow to go to waste. The flow rate during priming is 10 mL/min.

Recommendation: Ensure that all solvents in solvent reservoirs A, B, C, and D are full and miscible.
Note: To prevent salts from precipitating in the system, introduce an intermediate solvent, such as water, when changing from buffers to high-organic-content solvents. Consult the solvent miscibility tables in the Solvent Considerations section of the system guide.

Ensure that the solvent reservoirs contain sufficient solvent for adequate priming and subsequent operation of the system, and that the waste container can hold all the used solvent. For example, at 10 mL/min, priming for two minutes uses about 20 mL of each solvent.

Warning: To avoid spills, empty the waste container at regular intervals.
Requirement: Prime all solvent lines with solvent to ensure that the degasser and the gradient proportioning valve function properly.
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