Setting up solvents

Mobile phase solvents and the seal wash, needle wash, and purge solvents are set up before running equilibration.

Warning: To avoid personal contamination with biologically hazardous or toxic compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Required tools and materials

• Chemical-resistant, powder-free gloves
• Protective eyewear
• Clean solvent bottles

To set up solvents:

1. Prepare solvents in the clean bottles as required for the method.
   Note: There can be up to four mobile phase solvent lines, identified by the system as A, B, C, and D. Pay particular attention to how the solvents are lettered in the project because their identifiers determine which solvent line to connect to each bottle.

   The seal wash, needle wash, and purge solvents have dedicated lines, but it is possible for a method to specify filling a bottle with the same preparation as a mobile phase bottle.

   If necessary, obtain the solvent specifications for the method from the SOP or the Empower project.

2. Rotate each of the solvent bottles:
   1. Remove the cap and solvent line tube from the bottle that is being replaced.
   2. Remove that bottle from the tray.
   3. Pass the tube whose identification tag corresponds to the solvent through the opening in the replacement cap and install the cap on the bottle.
   4. Place that bottle on the tray as shown in the following figure.

   

3. From the touchscreen, set up initial priming of the solvent lines as described in Equilibrating the Alliance iS HPLC system.
   Note: If it is necessary to prime solvent lines outside of the equilibration workflow, return to the Home screen and select the appropriate workflow on the Setup > Solvents screen.

See:

• Priming the seal-wash system
• Priming the pump
• Priming the sample manager
• Solvent considerations
• Replacing solvent bottle filters