Reduce the risk of tubing connection leaks by following Waters recommendations. Also, ensure that appropriate and properly tightened fittings are used as described.
Three types of fitting assemblies are used within the system:
- Polyetheretherketone (PEEK), polymer-based
- Stainless steel (SST), gold-plated
- Tool-free fittings (TFF)
Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials. Consult the Safety Data Sheets regarding the solvents you use. Additionally, consult the safety representative for your organization regarding its protocols for handling such materials.
Warning: To avoid personal contamination with biologically hazardous materials, wear clean, chemical-resistant, powder-free gloves when performing this procedure.
Required tools and materials
- Chemical-resistant, powder-free gloves
- Protective eyewear
- 1/4-inch open-end wrench, for tightening or loosening SST (gold-plated) fittings with two-piece ferrules
- Permanent marker
When connecting tubing, heed the following recommendations for installing and tightening fittings:
- Before tightening compression screws, ensure that the tubing is fully bottomed in its connection port.
- For easier accessibility, use long compression screws to attach tubes to the injector and vent valve.
- Whenever you loosen fittings during maintenance, examine for cracks, stripped threads, and deformations.
- Whenever you loosen or replace fittings during maintenance, perform the solvent manager leak test (see your system's online Help).
- Except for those that are tool-free, do not reuse SST fittings more than six times.
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