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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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System preparation

Priming the system

Priming the system
Topic LCI-USG-0043, Last Updated 11/08/2024

After powering-on the system, you must prime it before the system is ready for use.

Requirement: You must prime the system after starting it, as well as after changing the mobile phase, after changing the sample needle, and after the system is idle for four hours or more.
Requirement: You must install a column before priming the system. See Installing the column.
Recommendation: If you are introducing new solvents, prime them at 4 mL/min for seven minutes. Alternatively, prime the solvents at 4 mL/min for three minutes. Ensure that sufficient quantities of solvent are available for priming.

There are multiple ways to prime the system from the touchscreen:

  • Tap SETUP > Startup to prime all solvents, prime the needle wash and seal wash, and specify the solvent composition, flow rate, column and sample temperatures, and needle characterization for your next system startup.
  • Tap SETUP > Solvents > Prime Mobile Phase Solvents to prime the pump.
  • Tap HOME, tap the solvent bottle icon, tap a mobile phase condition card, and then tap Prime Solvent to prime an individual mobile phase.
  • Tap SETUP > Solvents > Prime Sample Metering Pump to prime the sample metering pump.
Tip: From the touchscreen, you can select the Setup > Startup feature to prime all solvents, prime the needle wash and seal wash, and specify the solvent composition, flow rate, column and sample temperatures, and needle characterization for your next system startup. For details, see the touchscreen.
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