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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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Daily routine analysis

Equilibrating the Alliance iS HPLC system

Equilibrating the Alliance iS HPLC system
Topic LCI-USG-0196, Last Updated 11/08/2024

Equilibration prepares the system for accurate data acquisition. Run the equilibration workflow when the system is idle for four hours or more, or after changing the mobile phase or sample needle.

To equilibrate the system:

  1. From the touchscreen, click Commands to see the Commands view.
  2. Ensure that the Detector Lamp status is Lamp is on. Wait as long as necessary.
    Note: Allow 30-60 minutes for the detector lamp to warm up. For this reason, using the Detector Lamp command to turn on the lamp is preferable to turning it on during the following setup process.
  3. When the lamp status changes to Lamp is on, return to the Home view and tap Setup to see the Setup view.
  4. Tap Startup and follow the directions on the screen. Actions include:
    1. Priming the mobile phase
    2. Priming wash solvents
    3. Setting sample temperatures and checkpoints
    4. Switching on the detector lamp
    5. Setting flow rate, composition, and duration
  5. Review the settings on the Summary screen and tap Start to proceed with equilibration.
    The message System Startup In progress appears on the Status screen while equilibration is running. When equilibration ends, the system is ready for routine work.

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