Print Print
Alliance iS HPLC Systems User Guide
Contents Contents
Alliance iS HPLC Systems User Guide
Download PDF Download PDF
Solvent quality

Buffered solvents

Buffered solvents
Topic LCI-USG-0038, Last Updated 11/08/2024

When using a buffer, choose good quality reagents, filtering them through a 0.2-µm membrane filter.

Recommendation: To discourage microbial growth, replace 100% mobile aqueous phase daily.
Adjust the pH of aqueous buffers. Filter them to remove insoluble material, and then blend them with appropriate organic modifiers. After you use a buffer, flush it from the pump by wet priming using at least five system volumes of HPLC-grade distilled or deionized water.
Caution: When operating the Alliance iS Bio HPLC System above pH 10, ensure that you are using the optional high pH kit. For further assistance, contact Waters.

For shutdowns of more than a day's duration, flush the pump with a 20% methanol (MeOH)/water solution to prevent microbial growth.

See also: For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (715001307) on the Waters website ( www.waters.com).

Need Help? Connect with a Waters expert. 

Create Support Request

To avoid risk of personal injury and to prevent damage to laboratory equipment, always operate your Waters products in accordance with the applicable operational and safety information, your organization’s standard operating procedures, and local regulations.

Refer to the English version of this page for the latest information.

Back To Top Back To Top