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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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Sample manager features

Injection mechanics

Injection mechanics
Topic LCI-USG-0068, Last Updated 11/08/2024

The sample needle goes through a specific sequence for each injection.

During an injection, the following sequence takes place:

  1. The needle moves to the sample vial and aspirates sample from it.
  2. The needle carriage inserts the needle into the injection/wash port.
    Note: The entire injection port assembly rests on a spring and is guided inside a metal housing.
  3. As the needle is inserted into the injection/wash port, it presses against the seat and forms a high-pressure seal.
  4. The injection valve turns to start the injection.
  5. The wash pump washes the outside of the needle during the injection.
Figure 1: Needle seat assembly, cross view
Angled wash manifold
Sample needle support sleeve
Fitting
Seat
Seat port assembly
Seal cup locking nut
Compression spring
Needle wash housing

The following figure shows standard injection mode cycle time.

Figure 2: Standard injection mode cycle time definition
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