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Alliance iS HPLC Systems User Guide
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Calibrating the PDA detector

Mercury calibration

Mercury calibration
Topic LCI-USG-0207, Last Updated 11/07/2024

Mercury calibration uses a mercury-argon spectral calibration lamp to calibrate or recalibrate the instrument. Mercury calibration is typically performed at the factory but can be performed by qualified Waters field service engineers if necessary. Note that mercury recalibration should only be performed if erbium calibration fails or the prior mercury calibration parameters were wiped or corrupted.

A Waters field service engineer can perform a mercury calibration for the PDA detector from the system touchscreen.

Important:
  • Mercury calibration is to be performed only by a Waters field service engineer.
  • Impurities in the flow cell can affect wavelength calibration. Ensure that the flow cell is clean before you perform calibration.
  • This procedure can adversely affect spectral library matching and peak purity calculations.

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