Wash solvent recommendation

To maintain system condition and overall performance, follow this wash solvent recommendation.

The use of nonvolatile (solid salt) buffer solutions in wash solvents is permitted in limited concentration only. Generally, however, it is not recommended.

Solid, salt-based buffer solutions can dry and leave a salt residue that can scratch sealing surfaces, clog tubing, and damage the wash pump. Some applications control pH to promote sample solubility, which manifests in better peak shape and narrower peaks upon elution. Better sample solubility in the mobile phase also results in the sample components being less likely to adhere onto surfaces such as the stationary phase or the needle. If pH control is important to separation performance, consider it when determining the composition of wash solvent. For example, if acid (low pH) is required to keep the sample in solution during the separation, it is likely to be a required component of the wash solvent, necessary to dissolve the sample adhering to the needle's surface and to rinse the wash station.