Buffered solvents

Topic LCI-USG-0038, 最近更新 11/22/2024

When using a buffer, choose good quality reagents, filtering them through a 0.2-µm membrane filter.

Recommendation: To discourage microbial growth, replace 100% mobile aqueous phase daily.

Adjust the pH of aqueous buffers. Filter them to remove insoluble material, and then blend them with appropriate organic modifiers. After you use a buffer, flush it from the pump by wet priming using at least five system volumes of HPLC-grade distilled or deionized water.

Caution: When operating the Alliance iS Bio HPLC System above pH 10, ensure that you are using the optional high pH kit. For further assistance, contact Waters.

For shutdowns of more than a day's duration, flush the pump with a 20% methanol (MeOH)/water solution to prevent microbial growth.

See also: For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (715001307) on the Waters website ( www.waters.com).

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Buffered solvents